koeng

24 ms·

271.
by koeng 6y ago
I'm starting what could be thought of as a DNA cloning company. So sort of automation service, but for one specific application. > Are you doing some kind of mineral oil top layer to prevent evaporation The opposite: the process is
272.
by koeng 6y ago
I'd like that! If you want to get some readers for it, check out the DIYbio forums on google groups. They aren't as active as they were years ago, but there are still a bunch of knowledge people there.
273.
by koeng 6y ago
Yes I am really doing this at home with actual living biology. Garages are terrible for biotech because of contamination problems. I have a bedroom that I've sealed quite well with 2 box fans constantly running filtering the air (tape
274.
by koeng 6y ago
You can buy a ton of very expensive biotech equipment off of ebay for very little, which is what a lot of bootstrapped biotech companies do (plus gov-deals and other pharma auction sites)
275.
by koeng 6y ago
Sure. Right now, Opentrons rules with their open source robots, so liquid handling robots are pretty well handled. Xarm makes good arms for interfacing with Opentrons, so then it comes to everything else necessary to get to full automation.
276.
by koeng 6y ago
Maybe they were all govdeals type of people
277.
by koeng 6y ago
I've just started a bootstrapped biotech company in the last ~4 months, and it's definitely possible, but does depend on your niche. I'm lucky because I can live with my parents and convert my room into a lab, but normally yo
278.
by koeng 6y ago
I'm interested! Do you have a sign up page or something?
279.
by koeng 6y ago
Got a link or website? Sounds like a fun game.
280.
by koeng 6y ago
Using SQLite seems like a good way to get around some of the limitations that Restic has. Do you have any benchmarks against Borg and Restic? I'm looking for smaller single-host backup tools. I have approximately a ~1Tb Postgres databa
281.
by koeng 6y ago
Awesome package! I've been thinking about using datasette for some genomics data for a while. The value prop of "make nice visualization on top of SQLite" was very clear to me. Thanks for making an awesome project
282.
by koeng 6y ago
https://github.com/TimothyStiles/poly a modern synthetic biology library written in Go. Aiming at building some awesome stuff (codon optimization, synthesis optimization, RBS calculator, etc) that should really help in
283.
by koeng 6y ago
Anyone here tried both ledger and hledger? I read up on the docs, and would like to try one, but wanted to hear any colloquial experiences of usage before I dove in.
284.
by koeng 6y ago
I am a bigger fan of the hero stories where everyone is able to fight an epidemic themselves, instead of being subject to the whims of pharma / ineffective government policy. The moderna vaccine basically took a weekend to design, but
285.
by koeng 6y ago
I am building standardized and modular mass-distributed DNA toolkits. Two blog posts for context: https://blog.libredna.org/post/cheapdna/ https://blog.libredna.org/post/sporenet/ Websit
286.
by koeng 6y ago
Or you gene drive them.
287.
by koeng 6y ago
Not likely for nucleus - https://doi.org/10.3389/fmicb.2020.571831 Gemmata obscuriglobus is pretty interesting, and one could imagine how you go from it to nucleus https://en.wikipedia.org/wiki/Gem
288.
by koeng 6y ago
Link?
289.
by koeng 6y ago
How many git repos are published per hour to github? It probably doesn't matter, practically speaking, for you to build cool things. Similar to the results from pubmed. For a significant amount of synthetic biology, you don't real
290.
by koeng 6y ago
Is there any evidence of his theory of consciousness?
291.
by koeng 6y ago
This is cool! thanks and noted. I like the font, feels natural.
292.
by koeng 6y ago
Is being kind good for your health, or is having good health make you more kind?
293.
by koeng 6y ago
“ Residents are doctors in training, who have graduated from medical school.” It’s because you didn’t read the article. Residents are doctors in training, not just people living there - and they are interacting with sick people.
294.
by koeng 6y ago
How about the Q32-17? For $800, it sounds like a pretty decent server and I am interested.
295.
by koeng 6y ago
I'm wondering what kind of safety these would have. A 100 MW reactor in a single shipping container is crazy efficient - just curious what infrastructure around that would have to be built
296.
by koeng 6y ago
Whole plasmid. In my experience, Sanger is less accurate than Nanopore, especially since you’re getting 1x coverage per read, and in order to reliably get good coverage of sequence you need a primer every 600 base pairs. Consensus reads on
297.
by koeng 6y ago
Plasmid sequencing is what I’m using it for. Nanopore is the only technology (other than Pacbio) that you can solve the indexing problem with, which MASSIVELY lowers the cost per sample. Approximately $14 down to $2, which a LOT less manual
298.
by koeng 6y ago
Aligning doesn’t, but basecalling seriously does. The best base callers are still big machine learning models that take quite good GPUs
299.
by koeng 6y ago
Yea, you can use it for COVID19 diagnostic tests. I did that back in April-ish for a project before they redirected the Nanopore reagents to commercial COVID testing. Yes, it could be used to build a database, so long as you have a good amo
300.
by koeng 6y ago
They’re making the Smidgion which can directly connect to smartphones. The minion needs a USB connection tho, which is the $1000 one. The raw data is actually pretty intense to decode - it took my 4 core i7 processor (2016) about 30 hours t

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