Whole plasmid. In my experience, Sanger is less accurate than Nanopore, especially since you’re getting 1x coverage per read, and in order to reliably get good

Whole plasmid. In my experience, Sanger is less accurate than Nanopore, especially since you’re getting 1x coverage per read, and in order to reliably get good coverage of sequence you need a primer every 600 base pairs. Consensus reads on Nanopore aren’t that bad, and you can automate them way better than Sanger. I haven’t found any issue using them - IMO the “it isn’t accurate” stuff isn’t very accurate. Also, base pair resolution is bad on Nanopores primarily at homopolyer stretches. Guess what you can’t synthesize in the first place? (Edit: I’ve done Sanger sequencing on about 3,000 plasmids at once before for high throughput cloning, and that SUCKED.)