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I'm starting what could be thought of as a DNA cloning company. So sort of automation service, but for one specific application. > Are you doing some kind of m
by koeng 6y ago
I'm starting what could be thought of as a DNA cloning company. So sort of automation service, but for one specific application.
> Are you doing some kind of mineral oil top layer to prevent evaporation
The opposite: the process is pretty much based off of dessication (plus a little bit of tube opening by the bots for important chemicals). For thermocycling stuff, I'm planning on just using Opentrons module with the reusable seals. I really wanted to find a way to use reusable seals everywhere, but I just couldn't think of a way to do it well robotically. Thankfully, I don't need to convince the regulatory bodies of anything, since I'm just doing DNA cloning.
> We have an opentrons that we bought couple years ago
If you need to do multichannel stuff, do yourself a favor and go buy Gen2 multichannels and toss your old Gen1. The Gen1 multichannels are hilariously unreliable, but the Gen2 are pretty solid. Love those bots overall though - I got two of em so I can work in parallel!
- daemonk 6y agoDesiccation is an interesting approach. I don't see it as being used for every step of a reaction though? It would take too long to wait for desiccation between every step? And depending on the buffers you are using, you might be increasing the salt concentrations after you re-constitute? I guess you can take that into account when you add the reconstitution buffer. Are you open to discussing some kind of consulting arrangement between you and my company? If so, how do I reach out?
- koeng 6y ago> It would take too long to wait for desiccation between every step? Desiccation is just for the storage steps. Otherwise I just plan on passing liquids around, compensating for any evaporation. > Are you open to discussing some kind of consulting arrangement between you and my company? Sure! Contact me at koeng101 [@] gmail.com and we can talk