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Yes I am really doing this at home with actual living biology. Garages are terrible for biotech because of contamination problems. I have a bedroom that I've s
by koeng 6y ago
Yes I am really doing this at home with actual living biology.
Garages are terrible for biotech because of contamination problems. I have a bedroom that I've sealed quite well with 2 box fans constantly running filtering the air (tape a 20x20 filter to a box fan and it works great). When I work with media or anything I also use a flame. I still have some problems with contamination, but it is manageable.
There are also biological ways to get around contamination problems. So long as your media is clean, if you use a strain like Vibrio natriegens, it just outgrows literally everything, so it's not that big of a worry.
I have a few ruuvitags + a raspberry pi to monitor the temperature, and I live in Coastal California, so we don't have that much fluctuation. It hasn't mattered for my experiments, anyway, which are mainly just DNA cloning.