88e282102ae2e5b

8 ms·

31.
by 88e282102ae2e5b 9y ago
In theory, yes, but delivery of the CRISPR enzyme to all the infected nuclei is still an unsolved problem. Also, it doesn't cut its target every time it does get in - some of the protein is inactive (probably just broken/malformed
32.
by 88e282102ae2e5b 9y ago
There's a guy at my work who has a mild stutter. He starts every talk out with something like "My name's such and such, I have a mild stutter so don't let that bother you" and then immediately starts his talk and ne
33.
by 88e282102ae2e5b 9y ago
I've only looked into this a little bit, but I think one problem is that you're limited by either nitrates or phosphates, depending on whether the algae are being grown in ocean water or fresh water. Either way you have to supply
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by 88e282102ae2e5b 9y ago
I just keep the paper towel I used to dry my hands to cover the door handle as I open it, and only then throw it away.
35.
by 88e282102ae2e5b 9y ago
Maybe this could finally bring the use of cryptographic signatures into the mainstream. An additional incentive might be that they could even be used to "cheat" when unfavorable news came out: an organization that routinely signs
36.
by 88e282102ae2e5b 9y ago
I legitimately don't see which guideline this violates and I thought it was a good question, even if it turns out that the case for paying off the debt is strong.
37.
by 88e282102ae2e5b 9y ago
Though anecdotal, the one time I tried to restore my data from a duplicity backup (aside from when I initially started using it), I got a stack trace with an unhandled KeyError. I was never able to find out why the error happened or how to
38.
Young Again: How One Cell Turns Back Time(nytimes.com)
3 points by 88e282102ae2e5b 9y ago|0 comments
39.
by 88e282102ae2e5b 9y ago
The viruses used to deliver CRISPR in the past are not able to replicate. They're so small that you have to remove all of the viral DNA in order to even fit your CRISPR-coding genes inside.
40.
by 88e282102ae2e5b 9y ago
Cells need to be able to control how much cholesterol they have (cholesterol, notably, makes up about 25% of the cell membrane). They can either synthesize it themselves, or import it from the blood. The system for importing cholesterol can
41.
by 88e282102ae2e5b 9y ago
It's not like CRISPR didn't pan out - it's just that biology takes a long time to understand. Cas9 was only characterized 5 years ago. It's now routinely used in all sorts of research applications and clinical trials for
42.
by 88e282102ae2e5b 9y ago
Personally, If I were picking the assumptions and hyperparameters, I'd end up with the golden age being seasons 3 through 8 :)
43.
by 88e282102ae2e5b 9y ago
I'm definitely getting into hair-splitting territory, but I think it's important to not conflate modifying a protein with fusing two proteins. It would be easy to come away from the GP and think that scientists have the ability to
44.
by 88e282102ae2e5b 9y ago
So many reasons! * In order to get CRISPR to fit inside a virus you have to remove all the viral DNA, so it can't reproduce. * Even if you could make such a virus, there would be enormous selective pressure to ditch the CRISPR p
45.
by 88e282102ae2e5b 9y ago
> they modified the CRISPR associated DNA editing enzyme, cas9 This is incorrect. They modified a separate enzyme that is fused to cas9.
46.
by 88e282102ae2e5b 9y ago
I've confronted it plenty of times, and for my use cases it's not really an issue. Many numpy functions release the GIL, so by keeping my performance-critical stuff in numpy it's just not an issue for me. I sometimes split ta
47.
by 88e282102ae2e5b 9y ago
I didn't mean to seem petty, it's just that going back and forth between zero-based and one-based languages just added extra mental overhead (admittedly this was with Fortran, so there may have been other issues).
48.
by 88e282102ae2e5b 9y ago
I did read the post. > but rather it's first-in-class domain-specific packages. You'll note I didn't cite lack of libraries as a reason I'm reluctant to use Julia. I agree that a solid ecosystem is critical for adopti
49.
by 88e282102ae2e5b 9y ago
Is Julia really that much more performant than numpy?
50.
by 88e282102ae2e5b 9y ago
It's great and all, but I can't justify switching languages for minor improvements over Python + numpy/scipy. I'd be abandoning: * My deep knowledge and experience with Python * My entire codebase * The ability t
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by 88e282102ae2e5b 9y ago
Genuine question: with an extremely strong password on the private key, what's wrong with just storing it on an external hard drive somewhere?
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by 88e282102ae2e5b 9y ago
I would argue that Xubuntu is almost grandparent-ready.
53.
by 88e282102ae2e5b 9y ago
The initial meeting with the IRB where they proposed ridiculous and irrational changes to the consent forms was strikingly reminiscent of every conversation I've had in a third world country where someone was asking me for a bribe, but
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by 88e282102ae2e5b 9y ago
I'm glad I did it, but it was definitely more work than I was expecting.
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by 88e282102ae2e5b 9y ago
Well, you wouldn't want to modify a gene that isn't well-characterized, sure. But there are some things that are known with a great deal of certainty.
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by 88e282102ae2e5b 9y ago
I don't think there's any need for a cui bono line of thinking. There are biologists who have nothing to gain who thought this paper was lousy. For example, every single biologist in my department that I spoke to, and every biolog
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by 88e282102ae2e5b 9y ago
This paper was incredibly sloppy and has been widely dismissed by scientists in the field.
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by 88e282102ae2e5b 9y ago
This whole concept seems like a combination of a desperate wish to be living in a better world and the need to seem interesting at parties. When there's a compelling reason to get such devices you won't have to ask people what the
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by 88e282102ae2e5b 9y ago
It seems that for the glamor journals in biology it definitely is broken, at least for click-baity manuscripts like this. This paper was immediately shat upon by everyone in my department. There's a running joke often said when things
60.
by 88e282102ae2e5b 9y ago
Some of my colleagues speculated that this was not the intended study. Originally, they might have tried to delete a gene, but the expected phenotype didn't show up, which would have put an end to that line of research. But instead of

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