skosuri

45 ms·

151.
by skosuri 11y ago
Sorry, it's short for basepair (bp) of DNA (eg a, c, t, or g). Currently it costs about ten cents a bp to synthesize a particular stretch of sequence from one of the companies in the article (dime a base). An average protein can be enc
152.
by skosuri 11y ago
Synthesis is still quite expensive at a penny a base, much less a dime a base. To give a comparison to sequencing, costs are about a penny a megabase (~7 orders of magnitude cheaper). We have a long way to go. Even for a small bacterial ge
153.
by skosuri 11y ago
Wow, lots of moving parts, but it seems to work great. Actual paper here: http://rdcu.be/eHrW
154.
by skosuri 11y ago
I wasn't talking about copies of the same sequence. Agilent for instance makes 1M oligo arrays, and you can get these at 200nt each with arbitrary sequence. That's 200M bp of synthetic DNA spec'd to design on a single chip.
155.
by skosuri 11y ago
Perhaps they mean synthetic genes from the major market players. It's definitely not 10% of all DNA synthesis. For example, oligos produced on microarrays alone can produce this much DNA on a single chip. I'm assuming they mean
156.
by skosuri 11y ago
Oh interesting. We should definitely talk at some point. You in LA anytime soon?
157.
by skosuri 11y ago
Our lab is working on ways to screen large libraries of designed proteins for engineered orthogonally interacting libraries. The thought would this would be a start of a modular resuasable library for molecular self-assembly. If you are in
158.
by skosuri 11y ago
It's a pretty awesome time to be a biologist. Things are happening so quickly and profoundly. In sequencing alone, imagine having 30 years of Moore's law progress in 5 [1]; that just happened and we are still catching up to the co
159.
by skosuri 11y ago
Perhaps it's anecdotal, and there were many that were pretty high energy in the sense they did act like a little puppy. They are ridiculously cute. From what I saw (was told), it depended on how long they were selected for and there w
160.
by skosuri 11y ago
I got a tour of this place a few years ago when I was in Novosibirsk for a genomics conference. I'll see if I can dig up the pictures and videos, but a few observations. 1. The different body characteristics (fur coat, ribs, ears, et
161.
by skosuri 11y ago
I walk 2.5 miles everyday from UCLA to my place in West LA. That said, everyone looks at me weird.
162.
by skosuri 12y ago
The idea and implementation of gene drives have been around for a while since the development of zinc finger nucleases ~20 years ago. The issue has been they were hard to do. Now with CRISPR's it has been remarkably easier. Interesting
163.
by skosuri 12y ago
I went to a startup after my phd for a few years, came back and am now a prof. I can't say I was very successful in industry. It happens, it's fairly rare, but it is definately becoming more common
164.
by skosuri 12y ago
That's a peculiarity of stanford; but it's like $200/year. At UCLA we have something similar called an infrastructure fee which is about the same amount a year. Importantly, this is not something they are responsible for payi
165.
by skosuri 12y ago
It's not happening as far as I know (I'm faculty at UCLA and employ several postdocs).
166.
by skosuri 12y ago
Stanford has always been pretty good with postdoc pay, and postdocs never paid tuition. Pretty much all of these schools and mine (UCLA) are on similar levels. At all UC's postdocs are unionized as well given additional protections (li
167.
by skosuri 12y ago
Really, that is surprising, because it is disallowed. Stanford, like all UC's where I am at, have salary minimums. Stanford's minimum is higher than the NIH levels [1]. It would be hard to pay someone under the table, so I don&#x2
168.
by skosuri 12y ago
Can someone explain to me how Theranos is worth $9 billion? Everytime I hear about them, their "technology" sounds so sketchy that it's hard to think it is not a scam. Is there so much promise behind closed doors that I'
169.
by skosuri 12y ago
I am an author on the 2012 paper.
170.
by skosuri 12y ago
ridiculously expensive, slow, not random access, not re-writable.
171.
by skosuri 12y ago
Read and write were a couple of days each. Would be faster now. That said, we did 650kB; the article is misleading as they are talking about copies of the same information.
172.
by skosuri 12y ago
Depends on how you store it, but if it's dry it's much much longer. e.g., see here: http://onlinelibrary.wiley.com/doi/10.1002/anie.201411378/ab...
173.
by skosuri 12y ago
Yeah, the article isn't great. We go through how we calculated densities; we didn't use hard drive enclosures, but did include thickness for HD platters in calculating density. In general, magnetic tape mostly wins as they are s
174.
by skosuri 12y ago
We are doing things more dense now, especially w/ a new project w/ OK Go; but it would be a 2x gain in density, which isn't that big of a deal.
175.
by skosuri 12y ago
I think when we were doing the work it was largely philosophical for us to. The project was pretty simple practically.
176.
by skosuri 12y ago
Largely agreed, though I think information storage in sequenced polymers in general is fairly interesting, but we are long ways off. Also, there are applications for DNA storage that are somewhat interesting when weight becomes very import
177.
by skosuri 12y ago
This is pretty old, and not sure why it's here again today. That said, I was an author on the paper, and I'm happy to answer questions during a useless meeting I have to attend in 30 minutes.
178.
by skosuri 12y ago
Why would any commercial entity invest in things like drug development, crop development, etc; if the moment they actually bring something to market it's trivially copied and sold at cost?
179.
by skosuri 12y ago
Yes I know, but not to do what you are implying. Specifically, why would farmers spray their non-GMO corn, soybean, canola, etc with Roundup? It would kill most of their crop/harvest.
180.
by skosuri 12y ago
What? Why would a farmer spray roundup on their crop that's mostly not Roundup resistant? That makes no sense; it makes even less sense to do this season after season.

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