nebfield

3 ms·

1. 1.▲
   by nebfield 4y ago

   Very small amounts are sampled, on the order of grams. I'm not sure exactly, I worked on the bioinformatics side of things. I think an Illumina MiSeq requires 50 - 500 nanograms of DNA to work well. Sampling and storage methods can sig
2. 2.▲
   by nebfield 4y ago

   The burden of proof for a coeliac disease diagnosis is quite high: a biopsy from an endoscopy that shows classic signs of damaged villi (from autoimmune mechanisms), or a blood test with extremely high tTG levels and a family history of the
3. 3.▲
   by nebfield 4y ago

   The paper uses 16S rDNA sequencing, which is a bit old fashioned now but it was a good method when the paper was published. The steps basically involve: 1. Extract all DNA from poop, normally using a kit that basically makes DNA stick to ti
4. 4.▲
   by nebfield 4y ago

   Were you tested for coeliac disease? Diagnosis can be difficult, and the standard blood tests don't always pick it up. It might be a good idea to avoid wheat, barley, rye, and oats to test for gluten sensitivity.