4 ms·
At a basic level, methods of combining oligos to produce long strands have been known for ages. The challenge is to be able to produce them with low enough err
by cge 4mo ago
At a basic level, methods of combining oligos to produce long strands have been known for ages. The challenge is to be able to produce them with low enough error, high enough yield, and enough freedom on sequence. Low error improves your yield, reduces the amount of purification and amplification needed, and lets you make longer strands. Sequence constraints can be significant, too, especially around repeats.
If you're talking about Twist's gene fragment product, they advertise that as maxing out at 5 kb. Most, if not essentially all, of that month delivery time is likely the combination, not the oligo pool production. I think the Sidewinder people are actually using Twist pools; they're doing up to 12.5 kb.
By comparison, we recently needed something in the 20 kb range, with a not-so-great sequence, and it was a multi-month process to have a company produce it.
- kardianos 4mo agoYes. Whole genome sequencing has... some limits. CYP2D6 for instance is an important gene address, yet is rather hard to sequence do to its many copies and minor mutations. If you don't use targeted copy callers, it can be hard to correctly sequence in WGS.
- bonsai_spool 4mo ago> Yes. Whole genome sequencing has We're speaking about gene synthesis, not about DNA sequencing
- dnautics 4mo ago> not-so-great sequence are the limits on not-so-greatness for sidewinder known?
- amirhirsch 4mo agohttps://ansabio.com/ https://ansabio.com/ advertising 50 kb
- LarsDu88 4mo agoHow is ANSA bio? I just got hit up by a recruiter for this company at random, and now I see them mentioned on Hackernews
- amirhirsch 4mo agoIt's a sign! I'm too biased to give you advice.
- LarsDu88 4mo agoYou work there?
- amirhirsch 4mo agothe founder and i lived together at MIT. definitely go work there <3
- LarsDu88 4mo agoHmmmmm