3 ms·
The problem is Oxford Nanopore data has about a 30% insertion and deletion error rate. While PacBio is less than 1% indel and substitution error. Don't get me
by searine 9y ago
The problem is Oxford Nanopore data has about a 30% insertion and deletion error rate. While PacBio is less than 1% indel and substitution error.
Don't get me wrong, the Minion is amazing, but it can't compare to PacBio in terms of quality... yet.
- bsmrea 9y agoThe error rate of recent nanopore data is much less than 30%. See here for a recent benchmark: https://github.com/rrwick/Basecalling-comparison https://github.com/rrwick/Basecalling-comparison
- searine 9y agoInteresting, I haven't tried it on the newest chemistry but last time I did a flowcell (earlier this year) that was the INDEL error rate we achieve. Substitution error rate was very low, but you should evaluate INDELs specifically, rather than just identity like you did on your git, because it is the major drawback of this technology.
- bsmrea 9y agoJust to be clear this isn't my analysis - and yes most of the errors here are due to indels.