marsdentech

3 ms·

1. 1.▲
   by marsdentech 5y ago

   You might be interested in reading about "chaperone proteins"!
2. 2.▲
   by marsdentech 6y ago

   A single flow cell contains a few thousand pores (I think this is what you mean by "holes") that are all at different stages of passing different molecules, with signal data being captured from a few hundred at any given time. In
3. 3.▲
   by marsdentech 6y ago

   I used to run a department at a biotech where ~50% of our data came from MinIONs (although, that said, I'm a bioinformatician, rather than a molecular biologist), so I can answer your questions. For (a.), you can for sure "batch&q
4. 4.▲
   by marsdentech 6y ago

   This is a common, and often justified, though not always fair, criticism. MinIONs have an error rate of around 10% for _any given base_. Moreover, these errors aren't entirely independent of one another, so if you struggle to sequence
5. 5.▲
   by marsdentech 6y ago

   It's a complicated issue; I tend to think of the error component of any one MinION observation as being a function of the k-mer in the pore at the time (i.e. the subject of the observation) and, with some decaying dependence, the seque
6. 6.▲
   by marsdentech 6y ago

   As others have said, you're reading a sliding window of k-mers over the target sequence; I think for the MinION k is presently 5. To answer your question directly, it struggles with homopolymer runs, not inherently because they're