jfarlow

37 ms·

121.
by jfarlow 9y ago
Very much. We've actually tried to capture some of those runtime variables within proteins and break down the programs into smaller compossible units. And start to keep track of which conditions are required for particular functionali
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by jfarlow 9y ago
In that regard, the protein programs exist and interact with each other in a runtime environment/OS that is ' physics '. Which is cool. Especially, because those programs, when run together, along with all sorts of other sma
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by jfarlow 9y ago
As someone building software to design genetic tools, this is actually a pretty good overview. It's analogies are pretty solid, even if they are just analogies. It's a well-described snapshot of the early phase of education wher
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by jfarlow 9y ago
Some other footage: https://www.youtube.com/watch?v=NuXHriwQB9g https://www.youtube.com/watch?v=8ZZs1pxqB9c
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by jfarlow 9y ago
And a capsid might be able to store a few proteins, or a few mRNAs. But a few mRNAs deposited into a cell could produce many thousand protiens.
126.
by jfarlow 9y ago
Congratulations! Very cool. The goopy proteinaceous environment ("Extra Cellular Matrix") between living cells has such a strong effect on the communication and structure of tissues. And using concepts like tension and adhesion as
127.
by jfarlow 9y ago
The ability to intelligently design these atomically-precise nanomachines will be an ever-increasing part of our technological development. These proteins are small enough to fit into the features of a modern processor, with a physical res
128.
by jfarlow 9y ago
The Deisseroth lab has a materials list of the proteins utilized to do these neural optogenetic studies [1]. And if you want to play with those proteins yourself or design your own, you can use our software to explore others' designs a
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by jfarlow 9y ago
> Renewable Energy from Engineered Microbes This has been done. Amyris [1], among those that survived the effort, and JBEI from a quasi-academic approach [2]. From first principles, it just is not a good business proposition to compete
130.
by jfarlow 9y ago
> Cas-9 damages the DNA of some cells less because the the mutation was already there. The cells with no cas-9 are noise because they were never treated. If the mutations were already there, then control in figure 7 in Lonowski et al. wo
131.
by jfarlow 9y ago
I understand your hypothesis that Cas9 only kills the cells that do not have the particular mutation and itself is a negative selection against the undesired genetic sequence. I do not believe the basic numbers and statistics of the rate o
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by jfarlow 9y ago
Pretty cool visualization. Single molecule work like that is challenging to do properly - but when done properly gives a kind of visual proof to a hypothesis that is hard to controvert. Wild-type Cas9 cuts genomic DNA, and when it does, th
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by jfarlow 9y ago
There are a LOT of papers out there about CARs [1], how they work, and what their effects are. The DNA sequence used here has been effectively frozen for quite some time as it runs through all sorts of examinations. Further, your link is t
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by jfarlow 9y ago
And congratulations once again. This kind of therapy is a big deal because of just how different it is from standard 'drugs'. This is a precise and intentional genetic change to a patient's own immune cells to provide it a
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by jfarlow 9y ago
It is a bit of a simplification - but not by that many orders of magnitude ;). The really hard part (like small molecules) is the R&D to come up with that sequence. Unlike small molecules though, most people have the technological pro
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by jfarlow 9y ago
Open Insulin @ Counter Culture Labs: https://experiment.com/projects/open-insulin If you want to look at templates for your own immunotherapy, CRISPR, optogenetic tools etc.: https://serotiny.bio/notes&
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by jfarlow 9y ago
I have built a company for designing proteins for use in therapeutic synthetic biology (CRISPR/CAR/SynNotch/etc. systems). My undergrad background was more chemistry/physics/philosophy while my graduate school was
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by jfarlow 9y ago
It is happening. Hemophilia being cured at Marin Bio [1]. There are a bunch of therapies where Cas9 is being used by to therapeutically knock out or repair particular proteins. Indications currently targeted at Intellia: ATTR, Hepatitis B,
139.
by jfarlow 9y ago
Check out Trace Genomics [1] for a new company at QB3 that is taking a stab at quantifying what organisms are part of a particular farm's microbiome. They sequence the DNA of everything in a given soil sample, then figure out who&#x27
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New code for flipping a specific bit in a human genome(serotiny.bio)
4 points by jfarlow 9y ago|0 comments
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by jfarlow 9y ago
David Liu's lab last week published [1] an updated version 4 of their genomic "Base Editor" that is capable of switching a C to a T with sequence specificity within a living genome. I try to break down the sequence and compon
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How to flip a bit a specific bit in a human genome(serotiny.bio)
2 points by jfarlow 9y ago|1 comments
143.
by jfarlow 9y ago
David Liu's lab last week published [1] an updated version 4 of their genomic "Base Editor" that is capable of switching a C to a T with sequence specificity within a living genome. I try to break down the sequence and compon
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Code behind flipping a specific bit from C to T in a human genome(serotiny.bio)
3 points by jfarlow 9y ago|1 comments
145.
by jfarlow 9y ago
GFP and other fluorescent proteins now provide the basis of most debugging done in genetic development. The fluorescent proteins are so robust, straight-forward, and easily analyzable that they're used as console flags for 'is it
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by jfarlow 9y ago
These kinds of genetically designed therapies will require some major retooling of infrastructure, capital, regulation, etc., but are fundamentally cheap and curative - for mis/dis-regulation of the body's own systems. Ultimately
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by jfarlow 9y ago
Kite's $12b acquisition by Gilead [1] on Monday may be a harbinger of anticipated success of these kinds of biotherapeutics. Given the modularity of these CAR systems, I'd expect to see a cascade of more approvals as the therapeut
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by jfarlow 9y ago
Arbitrary biological i/o; stimuli and responses can be designed and linked, sampling from anything the world of life can currently do. Age (see Constitutional Article 2.1, 14th US Amendment). Memorizable strings of data worth billions
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by jfarlow 9y ago
And yet the supermarket is very much possible only because of the technologies behind planes, antibiotics and snapchat. It's curious. I think the hard part, as noted, is not necessarily the materialization of 'new tech', but
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by jfarlow 9y ago
'Cancer' is a huge umbrella term. Some cancers we know very well, many we do not. The one's being addressed by these therapies are well enough understood to target therapeutically. Some like cervical cancer, skin and lung

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