flobosg

65 ms·

901.
by flobosg 6y ago
If you liked the subversion aspect of NGE, then Puella Magi Madoka Magica might be up your alley. EDIT: Add Revolutionary Girl Utena to the list, although it might be considered somewhat repetitive and way too symbolic.
902.
by flobosg 6y ago
It’s probably uncommon, but hackers in the hospitality industry are not unheard of. See for example jwz and DNA Lounge.
903.
by flobosg 6y ago
I remember having looked at BASIC code without any English or programming knowledge and having my attention caught by the GOTO statements since it was the most Spanish-looking word around.
904.
by flobosg 6y ago
It seems that the author is not a native Spanish speaker, or at least not an idiomatic one.
905.
by flobosg 6y ago
Other Spanish-based programming languages: * PSeInt ( http://pseint.sourceforge.net/ ) * Pauscal ( http://www.pauscal.com.ar/ * IIRC, Logo has some Spanish interpreters
906.
by flobosg 6y ago
Connect to it via a Gemini[1] client or use a web proxy: https://portal.mozz.us/gemini/biomimetic.me/subleq.gmi [1]: https://gemini.circumlunar.space/
907.
by flobosg 6y ago
He is probably referring to this: https://en.wikipedia.org/wiki/The_Proms#Last_Night_of_the_Pr...
908.
by flobosg 6y ago
The words in the text have soft hyphens[1] that allow word breaking. [1]: https://en.wikipedia.org/wiki/Soft_hyphen
909.
by flobosg 6y ago
FWIW, the Elementary app started as a Geary fork, but if I’m not wrong it got a rewrite a while ago.
910.
by flobosg 6y ago
Seconding the Mailspring recommendation, but be aware that it requires a Mailspring ID. There’s some progress, though, with a telemetry-free fork[1] (that’s the version I’m using right now) and plans to make the ID optional in the official
911.
by flobosg 6y ago
To add to that, since 2017 Punta Arenas has its own time zone apart from the rest of continental Chile. It uses summer time (UTC-3) the whole year.
912.
by flobosg 6y ago
> Biology is an evolved machine. “Evolved” meaning a tinkered, duct-taped, convoluted, purposeless, billion-year old Rube Goldberg abomination of a machine. Any analogy with a man-made device will, at best, be incomplete.
913.
by flobosg 6y ago
Speaking of Michael Levin, I find this lecture of his fascinating: What Bodies Think About: Bioelectric Computation Outside the Nervous System - https://www.youtube.com/watch?v=RjD1aLm4Thg
914.
by flobosg 6y ago
Your post brings to mind Christian Bök’s “Ubu Hubbub”: https://www.lyrikline.org/en/poems/ubu-hubbub-10304 https://twitter.com/christianbok/status/1286894344566267907 Edit: He also likes
915.
by flobosg 6y ago
Happy to see Anna von Hausswolff mentioned here! Dead Magic is such a great album.
916.
by flobosg 6y ago
The mechanism of base complementarity is identical in both cases. If a modified uracil complements an adenine in tRNA, it will complement an adenine in the RT primer or an adenine being added to it.
917.
by flobosg 6y ago
While not completely equal to the naturally occuring bases, the modified bases in the vaccine mRNA need to be able to complement to the non-modified ones present in tRNA anticodons during translation. If they can pair to their corresponding
918.
by flobosg 6y ago
Since it is literally plain text (as opposed to HTML) it will default to the monospaced font set in your browser.
919.
by flobosg 6y ago
I wouldn’t call it extremely simplified; you can go simpler: https://science.sciencemag.org/content/351/6280/aad6253
920.
by flobosg 6y ago
Their codon compositions were optimized differently, but both coding regions translate to the same amino acid sequence.
921.
by flobosg 6y ago
Derek Lowe discussed it a few months ago: https://blogs.sciencemag.org/pipeline/archives/2021/01/11/rn... https://blogs.sciencemag.org/pipeline/archives/2021/02/2
922.
by flobosg 6y ago
> that's an arbitrary limit to separate proteins from enzymes Do you mean “to separate polypeptides from proteins”? Enzymatic activity has nothing to do with size. For example, one of the smallest enzymes in humans has 62 amino acid
923.
by flobosg 6y ago
The most significant diffs between both vaccines occur in the untranslated regions located around the protein coding sequence and will never be present in the actual spike protein. Regarding the protein coding region, because of the degener
924.
by flobosg 6y ago
The sequence can be changed and optimized for several reasons: * There are untranslated regions (UTR) that could influence the regulation or stability of the mRNA. * Since most amino acids are encoded by more than codon, the coding region f
925.
by flobosg 6y ago
A pairwise sequence alignment done with `needle` starts like this: BioNTech_Pfiz 1 -----------GAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAG 39 |||||.|.|..|||| ||| || Moderna
926.
by flobosg 6y ago
I think you meant https://www.ebi.ac.uk/Tools/psa/emboss_needle/ or https://www.ebi.ac.uk/Tools/psa/emboss_water/ ;-)
927.
by flobosg 6y ago
a) Yes, you can translate a mature[1] mRNA sequence, codon by codon, from the start until the stop codon, and it will give you the sequence of the protein it encodes. b) Coronaviruses have a RNA genome. Researchers extracted it from wild-ty
928.
by flobosg 6y ago
> So how different is the mRNA in the Moderna, BioNTech/Pfizer & CureVac vaccines? There are 1274 codon positions. 808 are identical across all 3 vaccines. 103 are unique to Moderna, 249 unique to BioNTech, 230 to CureVac https
929.
by flobosg 6y ago
> Is it simply that they took a sample of the vaccine and studied its composition using some standard machine/process? That’s exactly what they did.
930.
by flobosg 6y ago
If you have the time, it would be nice to transfer the data to the commonly used Genbank format.

More ›