entee

19 ms·

121.
by entee 7y ago
+1 the major advantage of RT-qPCR is it's a massively amplified test. Tiny amounts of virus can be detected. When you're detecting a patient's antibodies to the virus, the patient is producing massive amounts of it. That said
122.
by entee 7y ago
There's no stopping anyone from getting the primers (small pieces of DNA) and doing a qPCR yourself. You'll also need reverse transcriptase (the RT, converts RNA->DNA) and a way to purify RNA from a mix of cells/spit/
123.
by entee 7y ago
Yes, the "q" is often used instead of "real-time" to avoid confusion with RT, which can also be "reverse transcriptase". RT in these cases is the enzyme that converts RNA to DNA.
124.
by entee 7y ago
N95 respirators have a pore size on the order of 100-300 nm. Viral particles can be a lot smaller than that (5-10nm), but this coronavirus seems to have a 80-90nm diameter. That means a respirator might be able to stop the particles IF wo
125.
by entee 7y ago
I used to set up and run a large plate of qPCR (we didn't use the RT step in our particular use case) in an hour or so. Mind you this was for something like 200 samples. It took 2-3h to run the actual machine. If I was setting up such
126.
by entee 7y ago
RT-qPCR is indeed the common detection method right now. RT (reverse transcriptase) converts RNA->DNA, PCR (polymerase chain reaction) doubles DNA by replicating it over and over in heat cool cycles. To a first approximation, you get 2^n
127.
by entee 7y ago
Both the culture point and: "Peacetime CEO strives for broad based buy in. Wartime CEO neither indulges consensus-building nor tolerates disagreements." Feel off to me. You're always creating culture, and if you've got g
128.
by entee 7y ago
That's not likely. Italy has more hospital capacity per capita than we do [1], has lower cost hospital care, and has been more proactive about testing (almost 8x tests performed) [2]. More beds means less strain when things get bad. Lo
129.
by entee 7y ago
Thanks this helps a lot. One other Q: if the pellets spill, how dangerous are they from a radioactivity perspective?
130.
by entee 7y ago
The military may not have this problem but for civilian use I’d ask: how do you secure this? For example: how do you ensure someone with a semi truck doesn’t ram into it and spill massively radioactive stuff all over the place?
131.
by entee 7y ago
The article makes it very clear that he is speaking of the immediate forecast (next 3-6 months) not the longer term (6 months to 1 year or more). The virus is already all over the globe, so technically it's already edging near a pandem
132.
by entee 7y ago
I can’t imagine that with stacked seating and ladders that come out and block the aisle that you could evacuate a plane in 90 seconds or less. That’s the standard for the FAA: https://www.washingtonpost.com/local/traffi
133.
by entee 7y ago
I thought you had a good take till I got to the end and saw a conspiracy theory that to my knowledge has very little basis in fact. Simple explanations (species jump, evidence of similar viruses in adjacent species) are way more likely than
134.
by entee 7y ago
In this case I’m not sure if the reasoning for what molecules work would make sense even if we had an “oracle” to explain it. Why a molecule works is a complicated interplay of fundamental physics and emergent properties. The explanation is
135.
by entee 7y ago
Sure! To a first approximation they both provide software packages to pharma who then apply those to their efforts. In our case we are building an integrated system with a biochemical component and a computational component. We will then se
136.
by entee 7y ago
I share some of your skepticism, but I think this particular paper is worth a little less cynicism. It’s true that statistical analysis has always helped inform medicinal chemistry efforts. They used to be called QSAR ;) What’s different he
137.
by entee 7y ago
This paper shows what can be done when you carefully run an ML program alongside a wet lab experimental program tailored to feed back into the ML program. The results end up far more interesting than some recent "ML aided drug discover
138.
by entee 7y ago
Right but there were tons of used cars in 1996 too that were available at a similar discount. The tweets are incomplete in that they fail to fully explain how CPI adjusts internally for quality improvement, but if we buy that (I think it’s
139.
by entee 7y ago
Fair enough but I bet there were plenty of times before/after a round where they interacted closely.
140.
by entee 7y ago
Ellison hosted a big fundraiser for Trump literally yesterday and Obama regularly golfed with Ellison. I’m not usually a conspiracy theorist but it’s clear the guy is connected and it stands to reason it might influence the government posit
141.
New Analysis Challenges Stem Gender Equality “Paradox”(genderscilab.org)
1 points by entee 7y ago|0 comments
142.
by entee 7y ago
The two income trap you mention was the subject of an Elizabeth Warren book sometime in the early 2000s. It’s a real issue, I’m not sure how to solve it, but it seems like a different larger scale issue. Also at this point its a little lat
143.
by entee 7y ago
Not sure I follow. Given two mothers (gonna leave aside some of the baggage here, fathers can do child care, not all women/people are straight or fit in the conventional framework): Amanda wants to have kids but also wants to be a Supr
144.
by entee 7y ago
I agree with this and the subtlety of the OP’s argument. There is clearly a problem, there are clearly many contributors, I have personally seen The OP situation play out with my female friends/colleagues in STEM (and other “high power
145.
by entee 7y ago
Fair. I guess there could be a different model too: I pay a "subscription" which means I get x submissions per year for the institution or something along those lines.
146.
by entee 7y ago
We're combining a technology called DNA encoded libraries (DEL) with machine learning. DELs let you make very large numbers of compounds without losing track of each in a large mixture (1 million to 1 billion compounds in one tube). Th
147.
by entee 7y ago
I love the DOI->PDF single search, it's how it should be. I'd prefer a better more responsive format, (PDFs are still hard to read on a phone or less than huge tablet) but nothing is quite as good for, "just give me the da
148.
by entee 7y ago
Sci-hub has been critical to me as I developed the idea for my current startup. If it didn't exist, I could have gotten most of the papers from friends in academia, but it would have been dramatically harder and slower. I use it, I sup
149.
by entee 7y ago
That's wild, agreed that is out of step. I usually book fairly last minute so I haven't seen those prices personally, but I know they exist. I surely don't believe we should favor air over train.
150.
by entee 7y ago
You're right I think some are overdone. One example is the consultants taking a plane 2x a week (leave early monday, come back thursday eve, often with unconstrained distances). Maybe a bunch of those could be cut, though in that case

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