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PCR is central to short read sequencing... as well as Roche 454, and can also be used in some protocols in Nanopore. https://pmc.ncbi.nlm.nih.gov/articles/PMC3

PCR is central to short read sequencing... as well as Roche 454, and can also be used in some protocols in Nanopore. https://pmc.ncbi.nlm.nih.gov/articles/PMC3849550/ https://pmc.ncbi.nlm.nih.gov/articles/PMC3849550/ You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion https://nanoporetech.com/news/news-human-genome-minion

bonsai_spool 11mo ago
> PCR [...] can also be used in some protocols in Nanopore. Yes... and that is why I said that the presence of PCR does not imply short reads. > You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion https://nanoporetech.com/news/news-human-genome-minion THat's why I said that 5/20/30X coverage is possible in the appropriate research setting