2 ms·

There wasn’t enough information to tell (on my 1 minute scan) which nanopore kit was used, but the presence of PCR does not imply short reads. You can do nanop

There wasn’t enough information to tell (on my 1 minute scan) which nanopore kit was used, but the presence of PCR does not imply short reads. You can do nanopore PCR/cDNA workflows right up to the largest known mRNAs (13kb). Edit: I’m not sure if you’re saying that you can’t do a 5/20/30X genome on nanopore - that’s also not true. It only makes sense in particular research settings, of course.

zorgster 1y ago
PCR is central to short read sequencing... as well as Roche 454, and can also be used in some protocols in Nanopore. https://pmc.ncbi.nlm.nih.gov/articles/PMC3849550/ https://pmc.ncbi.nlm.nih.gov/articles/PMC3849550/ You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion https://nanoporetech.com/news/news-human-genome-minion
- bonsai_spool 1y ago
  > PCR [...] can also be used in some protocols in Nanopore. Yes... and that is why I said that the presence of PCR does not imply short reads. > You can sequence a human genome on a MinION - but you need to purchase 5 flow cells to reach 11X (if they are used correctly).. https://nanoporetech.com/news/news-human-genome-minion https://nanoporetech.com/news/news-human-genome-minion THat's why I said that 5/20/30X coverage is possible in the appropriate research setting