3 ms·
As a backdrop for reading this, electroporation is one type of transfection, which is a method to "artificially introducing nucleic acids (DNA or RNA) into cell
by pugworthy 3y ago
As a backdrop for reading this, electroporation is one type of transfection, which is a method to "artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection."
You can also basically squeeze cells to do this, as well as put them under higher hydrostatic pressure.
I also think I know the internal project name for something...
- staticautomatic 3y agoYou can use chemicals too.
- DoingIsLearning 3y agoIs there some state of the art Handbook (Springer Taylor&Francis type) that you could recommend on physical methods of Transfection that goes into the physics of it?
- flobosg 3y agoTransfection by heat shock is also a possibility. Anything that creates transient pores on the cell membrane should work, at least in theory.
- twic 3y agoPerhaps the most brutal way of introducing nucleic acids into cells is microinjection: you make a really, really fine needle, jab it into a cell's nucleus, and inject a DNA solution. It is incredibly painstaking work, does kill a lot of the cells, and making the needles is a black art, but it works for all sorts of cells, even those resistant to chemical methods, so it is sometimes still useful.
- patcon 3y agoWhile mostly used for plants, there's another method that's essentially shooting cells with bullets coated in genes https://en.m.wikipedia.org/wiki/Gene_gun https://en.m.wikipedia.org/wiki/Gene_gun