One thing to note is that the fluorescence images like https://www.nikonsmallworld.com/galleries/2023-photomicrography-competition/rodent-optic-nerve-head https

One thing to note is that the fluorescence images like https://www.nikonsmallworld.com/galleries/2023-photomicrography-competition/rodent-optic-nerve-head https://www.nikonsmallworld.com/galleries/2023-photomicrogra... are false color. In those systems, you typically excite a specific fluorophore, one at a time (once for each type in the sample), and read out the result with a monochromatic camera through frequency-specific filters. The final image is composited with the grayscale intensity mapped to color value. I don't think the microscopy images underwent sharpening, although I'm not certain. My friend who works in the field prefers to look at individual color planes in monochrome (no color at all) rather than the super-saturated composites.