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The general field is Epigenetics (gene expression based on environment), and within that there is research on how peptides act as modulators by silencing and un
by cjbgkagh 4y ago
The general field is Epigenetics (gene expression based on environment), and within that there is research on how peptides act as modulators by silencing and un-silencing different genes. You can get a list of known bioactive peptides by reading it from the DNA or by using mass spectrometry (there’s a proper name for this but it escapes me atm). So putting the two together you can have quite a bit of external control over gene expression.
- rolph 4y agoHPLC [1] or Electrophoresy [2] would be instrumental in isolating peptides based on mobility, and molecular mass. [1] https://en.wikipedia.org/wiki/High-performance_liquid_chromatography https://en.wikipedia.org/wiki/High-performance_liquid_chroma... [2] https://en.wikipedia.org/wiki/Electrophoresis https://en.wikipedia.org/wiki/Electrophoresis
- cjbgkagh 4y agoI was thinking of MALDI-TOF mass spectrometry devices used in Protiomics research. It’s a bit out of my field but I think it’s an improvement to HPLC.
- dekhn 4y agoProbably GC-MS? https://en.wikipedia.org/wiki/Gas_chromatography%E2%80%93mass_spectrometry https://en.wikipedia.org/wiki/Gas_chromatography%E2%80%93mas... Dunno how that would isolate the sample, rather than just analyzing it though
- cjbgkagh 4y agoI’m not involved in the process of acquiring the ‘omics data. I just analyze it and read papers from other people analyzing it. I think MALDI-TOF is the gold standard for proteomics.
- rolph 4y agomass based deflection and deposition. isolation,and analysis are two different activities, many instruments are capable of both modalities, depending on technique. the problem is selecting a procedure that will not induce confounding artefacts of chemical, or physical interaction w the subject molecular entity.