> how much about protein denaturation do we need to know before protein structure prediction is useful ? Denaturation is not all that significant. Physiologic

> how much about protein denaturation do we need to know before protein structure prediction is useful ? Denaturation is not all that significant. Physiological conditions (i.e. pH, temperature, etc) tend to be maintained by homeostasis. Protein structure prediction is already incredibly useful. > what is the status of our current metabolic pathway simulations for example ? IMO we do not have the data to build useful metabolic pathway simulations. To do a good simulation you need to know (1) the proteins and metabolites involved, (2) their functions and key biophysical properties (ex. Km), (3) feedback / control elements. For most pathways we do not have (1). The data for (2) are mostly nonexistent, buried in literature, and / or unreliable. Same with (3).