3 ms·
> Then I would add the dideoxynucleoside triphosphate mixtures, and another aliquot of polymerase Ever thankful for heat stable polymerases.
by alextheparrot 6y ago
> Then I would add the dideoxynucleoside triphosphate mixtures, and another aliquot of polymerase
Ever thankful for heat stable polymerases.
- dekhn 6y agowhen he started, he didn't have heat stable polymerases- that came later. Instead you had to add more pol each step, which was a huge pain.
- alextheparrot 6y agoI know, just glad I worked in a molecular biology lab after Taq was discovered. My lab experience was akin to his first hopes — put the reagents in, place it in the thermocycler, and pick it up the next morning.
- dekhn 6y agoyou probably worked in a lab during the period where scientists, annoyed that PCR machines required purchase of licensed Taq, simply cloned and expressed their own and used it instead.
- alextheparrot 6y agoIf only I were so lucky, might've made me more thoughtful when planning experiments