3 ms·
That's fascinating, and I deeply admire the kind of science that involves developing new tools to find answers. Can I ask about the rationale motivating attemp
by pryce 7y ago
That's fascinating, and I deeply admire the kind of science that involves developing new tools to find answers.
Can I ask about the rationale motivating attempts to image DNA specifically - was it mainly that we were attempting to get better spacial understanding of the different molecular conformations it enters at different stages of the cellular cycle?
Or imaging DNA transcription? Or DNA polymerase in action?
or was imaging DNA this way an attractive goal because we already anticipated some particular applications?
- dekhn 7y agoimaging dna in this way should give high resolution, but if I understand the experimental conditions, they're not physically relevant, so would not be able to look at the molecular conformation of cellular DNA.
- deleted 7y ago[deleted]
- kurthr 7y agoThe imaging depth that I was aware of at the time (um) would have allowed imaging with the DNA is solution... so ostensibly introns and other physical attributes could have been imaged. Also 20 years ago we didn't have good automated shotgun techniques so everyone was trying to solve that problem.