3 ms·
I worked with Dr. Lutz about 20yr ago, helping to make some of the early magnetically tipped cantilevers for the effort at single atom NMR. It's interesting to
by kurthr 7y ago
I worked with Dr. Lutz about 20yr ago, helping to make some of the early magnetically tipped cantilevers for the effort at single atom NMR. It's interesting to me that the NYT shifted the nomenclature to MRI... although with microscopic scanning that is what they can achieve. It's not that weird because (at the time) the tip magnetic field caused a torus to interact with the cantilever when the atom(s) resonate, so they do have to deconvolve the effect to localize the atom and I assume that they use force feedback control to maintain position much like STM (Tunneling Microscopy). I probably would have called it something like NMM (Nuclear Magnetic-Resonance Microscopy), but hey I didn't make it work!
I'd note that the same group headed by Lutz achieved single electron ESR with a similar scanning methodology at least 15 years back so this was follow on work. It's still amazing and incredibly difficult... just imagine how you would produce something (a test sample) that you know will have only 1 magnetically resonant atom in the affected scan area!
At the time the hope was to use single atom NMR to image DNA, but I think that has largely been accomplished by other methods.
edit: The paper in Nature for those with access.
https://www.nature.com/articles/s41567-019-0573-x https://www.nature.com/articles/s41567-019-0573-x
- pryce 7y agoThat's fascinating, and I deeply admire the kind of science that involves developing new tools to find answers. Can I ask about the rationale motivating attempts to image DNA specifically - was it mainly that we were attempting to get better spacial understanding of the different molecular conformations it enters at different stages of the cellular cycle? Or imaging DNA transcription? Or DNA polymerase in action? or was imaging DNA this way an attractive goal because we already anticipated some particular applications?
- dekhn 7y agoimaging dna in this way should give high resolution, but if I understand the experimental conditions, they're not physically relevant, so would not be able to look at the molecular conformation of cellular DNA.
- deleted 7y ago[deleted]
- kurthr 7y agoThe imaging depth that I was aware of at the time (um) would have allowed imaging with the DNA is solution... so ostensibly introns and other physical attributes could have been imaged. Also 20 years ago we didn't have good automated shotgun techniques so everyone was trying to solve that problem.