3 ms·
>The error rate is stupidly high (somewhere between 10 and 20%) The Insertion/deletion error rate is 20-30%. The point mutation error rate is something 0.1-1%
by searine 9y ago
>The error rate is stupidly high (somewhere between 10 and 20%)
The Insertion/deletion error rate is 20-30%.
The point mutation error rate is something 0.1-1% (higher than HiSeq but not crazy high).
This means with a semi-decent reference genome you should be able to do re-sequencing fairly accurately. It also means, that in conjunction with HiSeq reads you can do cheap genome assembly, using the HiSeq reads for coverage, and the minion reads for scaffolding.
- comstock 9y agoDo you have a citation for this? Because this is not my understanding.
- searine 9y agoNo citation, just personal experience aligning the data and comparing it directly to a hiSeq run of the same DNA. I was able to get about 1-10% mutation rate, with a median of about 1.5%. Rate depending on quality of the run. In general it was on par with PacBio.
- daemonk 9y agoI can add to this with my anecdotal evidence. In my experience (looked at ~15gb of basecalled data total), there is a large amount of indel errors. The mismatch rate is much lower. But it's hard to calculate exactly the mismatch rate when the indel rate is so high.