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It looks like the lower bound for commercial whole genome sequencing is about 250 nanograms. To put this in perspective, you can get 4000 nanograms of DNA from
by bhickey 10y ago
It looks like the lower bound for commercial whole genome sequencing is about 250 nanograms. To put this in perspective, you can get 4000 nanograms of DNA from a 2 microlitre blood draw.
Your mental model of sequencing needs an update. :) Prior to sequencing, DNA is amplified via PCR. This dramatically increases the amount of DNA you have (2^30, iirc.)
- new299 10y agoYes, I've seen single cell preps too. So it basically just depends on your sample prep and what's practical (and what you can put into production). However you do it, as you say there's a bunch of amplification that happens prior to sequencing (currently).
- dekhn 10y agoPCR also amplifies the noise, and in a nonlinear way, especially with very small samples.
- viraptor 10y agoYou're talking about a clean blood draw though. I don't know how well it compares to dried cells you left somewhere for hours which are possibly mixed with many other people's cells. Do you have information about that?