eggie

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211.
by eggie 11y ago
> they're optimized for performing this task with larger strings on the order of thousands of times per second Bingo. And they use on the order of the genome size in RAM to do so. The FM-index is a helluva sequence search machine.
212.
by eggie 11y ago
But CRISPR/Cas9 is not open source. It is proprietary! A half billion year old natural system has been slightly tweaked and is now owned by the discovering groups. It is free for research use and for commercial purposes might still be
213.
by eggie 11y ago
Precise targeting of genomic cuts has been possible for some time, but more expensive: https://en.wikipedia.org/wiki/Zinc_finger_nuclease https://en.wikipedia.org/wiki/Transcription_activator-like_
214.
by eggie 11y ago
https://en.m.wikipedia.org/wiki/Mycoplasma_laboratorium
215.
by eggie 11y ago
Mostly, CRISPR/Cas9 reduces the cost of getting a custom endonuclease (molecular scissors that cut DNA at particular sequences). It is several orders of magnitude cheaper than alternatives, and it is also incredibly quick to set up! Th
216.
by eggie 11y ago
Learn Dvorak and save your hands (and maybe career). And type faster, in a few months.
217.
by eggie 11y ago
This is possibly the most reasonable piece I've seen linked on the topic. CRISPR/Cas9 techniques do not give us anything we couldn't do before--- after all there have been TALENs and zinc finger nucleases for quite a while. I
218.
by eggie 11y ago
Exciting but mundane. A lot has been invested but there have been very few useful results. It turns out the genome offers very little more than you already know from living in your body every day.
219.
by eggie 11y ago
You remind me of digital tools that are just coming into existence. Distributed autonomous corporations could organize most of the digital communication functions that the social media bubble is currently exploring. So, ethereum, Turing com
220.
by eggie 11y ago
Is that because you dislike facebook, or this kind of acquisition pattern?
221.
by eggie 11y ago
This is the relevant bit: > Fed has created abnormal market conditions by printing money and keeping interest rates low. Investors are looking for growth anywhere they can find it and tech companies are good targets - at these values, ho
222.
How the second tech bubble will burst(goldworth.com)
1 points by eggie 11y ago|1 comments
223.
by eggie 11y ago
> How many people have any real basis for knowing what Zuckerberg meant by those words? A line like that could mean almost anything. You'd have to have known him at the time. Also, how many people can truly claim never to have said
224.
by eggie 11y ago
I quit using facebook after I watched him exposing people on the site to everyone who happened to be hanging out in the computer lab (basement Harvard science center). He came off as incredibly inhumane. I got the impression that he feels o
225.
by eggie 12y ago
On the other hand, that which frightens elders which may hold the most promise for the youth. Your comment is a bit closer to home. I've spent the last decade aiming my life at exactly those things which frighten George.
226.
by eggie 12y ago
facebook's core offering is slowly walking towards the exit: http://www.google.com/trends/explore#q=%2Fm%2F02y1vz . Much of that valuable data stands to stale. (edit: how do i link.. oh man)
227.
by eggie 12y ago
See node.js streams.
228.
by eggie 12y ago
Cyclists aren't trying to "own" the lane any more than you are. Really, both of you own it, as citizens and road users. The reason for being in the lane is safety. In many situations, impatient people who feel they are impede
229.
by eggie 12y ago
Unfortunately the $1000 genome isn't really accessible unless you are inside of a research or clinical setting. Genomes at high-coverage still cost ~$6k, and likely will for the near future. This makes it very hard to begin utilizing
230.
by eggie 12y ago
On an XTen you could potentially do a few thousand genomes for that price... If they allowed it.
231.
by eggie 13y ago
There tends to be less overhead in TSV. Unless you want to represent text that has embedded tabs it seems unnecessary. It works with standard *nix tools. Not a bad compromise and part of the reason that people whose standard "file&q
232.
by eggie 13y ago
In science, a lot of people use ASCII and flat files. I used to really dislike it, but over time I understood that there are certain practical reasons to do this which deserve respect. Due to the volume and novelty of data that we work wit
233.
by eggie 13y ago
I have long told recruiters that I'd need something in the $250k range to even consider working as an engineer for a corporate tech shop. The work is brutal. You sell your mental life to hippie robber barons who write emails in all l