daemonk

9 ms·

1. 61.▲
   by daemonk 5y ago

   The flexibility of a graphdb translates to flexibility in writing your queries. And that's my main problem with graphdb. Query optimizations can be difficult. It is very easy to write a query that logically does what you want, but take
2. 62.▲
   by daemonk 5y ago

   I wonder if this will put selective pressure on weeds to evolve to look like crops.
3. 63.▲
   by daemonk 5y ago

   I have a phd and have postdoc'ed at a couple of places also. I am now in industry. Sure, there are boring bits. But I think what he is more looking for is being able to explore the space and more open-endedness to projects, which I do
4. 64.▲
   by daemonk 5y ago

   Hiring is a lot of the times more about finding someone that'll fit in your current team than about an individual with a lot of skill/enthusiasm. You don't sound very reliable and that would be my main concern. Even if you we
5. 65.▲
   by daemonk 6y ago

   There are a lot of numerical structures missing from this. Not sure if you can really advertise it as for data science without some kind of dataframe structure.
6. 66.▲
   by daemonk 6y ago

   Thanks for the response. I think protocol-bias is something that will be hard to control for. The collection kit, extraction method, library prep method can influence the results significantly from my experience. For example, we've see
7. 67.▲
   by daemonk 6y ago

   Are you guys depleting host or enriching microbial? Are you analyzing the data as a composition via log contrasts? Are you using available pipelines (kraken, metaphlan, etc) or something custom? Any plans on doing a functional pathway analy
8. 68.▲
   by daemonk 6y ago

   I have mixed feelings about at home allergy testing. I know that various clinical labs may have their own reference ranges for IgE/*. Combined that with a low amount of blood collected and sampling exposure time, the data would seem ve
9. 69.▲
   by daemonk 6y ago

   There are a lot of good work digging into the mechanism of how it causes cardiac fibrosis in rats. I don't think we should completely dismiss it just because it is in a non-human model. If anything, this study allows us to try to use t
10. 70.▲
    by daemonk 6y ago

    We've sequenced a lot of RNA in the past decade. A lot of that were extracted from neurons. I don't think we've seen evidence of this memory storing function through sequencing.
11. 71.▲
    by daemonk 6y ago

    I am not an expert obviously. But is this generally true in most situations? It just seems like maybe in this specific once-in-a-lifetime situation, maybe this doesn't apply? The decision to freeze all trades seems more "neutral&q
12. 72.▲
    by daemonk 6y ago

    So the argument is that if they just froze trades (both buying and selling), then people who own many GME shares and want to sell would not be able to and there would be a subsequent backlash? Maybe? I am trying to compare the lesser of two
13. 73.▲
    by daemonk 6y ago

    They could have just completely frozen trades of these volatile stocks then? Instead, they only blocked out buying these stocks and still allowed selling. Did they not really think through the implications of this action?
14. 74.▲
    by daemonk 6y ago

    If you want CLIA lab certification, there are a number of rules surrounding cross contamination, which not sealing a plate of samples properly might be a red flag.
15. 75.▲
    by daemonk 6y ago

    I want to point out that it really depends a lot on what kind of lab it is. I think for R&D, buying cheap stuff off of ebay or labx is great. But if your goal is to eventually scale up and build a production lab, you will need to switch
16. 76.▲
    by daemonk 6y ago

    Desiccation is an interesting approach. I don't see it as being used for every step of a reaction though? It would take too long to wait for desiccation between every step? And depending on the buffers you are using, you might be incre
17. 77.▲
    by daemonk 6y ago

    I completely agree with your point about being good at software/hardware/biology is the key to running a successful production biotech lab because you can try to supplement various weak points with the other domains. I've bee
18. 78.▲
    by daemonk 6y ago

    This is something that's extremely annoying to deal with in a genomics context. Various formats in the field uses 0-based or 1-based and inclusive or exclusive.
19. 79.▲
    by daemonk 6y ago

    This is pretty cool. How does the storage format work? Would I be able to download storage chunks from s3 and somehow recover my files from chunks?
20. 80.▲
    by daemonk 6y ago

    Yeah, the combination of features at the right quality level makes it the best laptop I've ever owned.
21. 81.▲
    by daemonk 6y ago

    This really cool. The examples in github doesn't seem to show any defining of feature types (categorical, binary, continuous, etc). Is there automatic detection of feature type?
22. 82.▲
    by daemonk 6y ago

    Here is my take on this. This was a fun project: https://gist.github.com/damiankao/81b6ebd123b9ccf98e0e47f1dd... This is a naive probabilistic method that calculates probabilities of base change according to base posit
23. 83.▲
    by daemonk 6y ago

    It would probably be really important miniaturize this as much as possible to reduce reagent costs. Primary/secondary antibodies are not cheap.
24. 84.▲
    by daemonk 6y ago

    I can't get past the motion sickness. I wonder what percentage of the willing market also can't handle it.
25. 85.▲
    by daemonk 6y ago

    I think one of the guys who founded Moderna originally wanted to inject mRNA to induce pluripotent stem cells. It would be great if that can somehow work for this. Instead of using an adenovirus to insert the genes, we can inject mRNA to in
26. 86.▲
    by daemonk 6y ago

    For normal daily use, I prefer something that don't require too much messing around with. I don't really care that much about customization either. For work, I'll just ssh into a remote linux server. I don't necessarily
27. 87.▲
    by daemonk 6y ago

    There is a big difference in definition depending on what part of the term "epigenetics" you emphasize. Emphasizing "epi" means you are looking at architecture/mechanistic of chromatin and how they affect function.
28. 88.▲
    by daemonk 6y ago

    I guess if you are looking at this solely from the perspective of using it for gene editing and "curing" disease, I would agree that practical applications might be over-hyped. But it is still a great tool for research. Many model
29. 89.▲
    by daemonk 6y ago

    There is a commercial solution that fuses multiple materials on the fly as it feeds into the the printer: https://www.mosaicmfg.com/ From what I've heard, there is a lot of filament waste still. But the results looks d
30. 90.▲
    by daemonk 6y ago

    As much as unstructured data makes me uncomfortable, I think something like this is probably the future of data keeping.