daemonk

31 ms·

271.
by daemonk 11y ago
What do you mean by the primers can just well be amplifying the template? How does that explain knock-ins without an actual insertion? And there are plenty of knock-in CRISPR papers out there. Just literally search for "CRISPR knock-in
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by daemonk 11y ago
But there are plenty of knock-in experiments where foreign DNA was put into the cut site. Are you saying a pre-existing mutant with the foreign DNA was used in those cases too?
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by daemonk 11y ago
How far removed from the purchasing chain is someone/organization deemed "morally clean"?
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by daemonk 11y ago
Says all first year phd students until their third year. I am currently a postdoc. It's a scam that academia perpetuates this idea to justify paying less.
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by daemonk 11y ago
Does this work with svg?
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by daemonk 11y ago
I think learning to do things in moderation is really the key here. I liked that the article is not vehemently chastising social media as the bane of modern civilization nor praising it as the second coming. There are pros and cons to adopt
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by daemonk 11y ago
Nice. There are already a couple of programs that do this I think, but maybe use AWS CLI to write a plugin for monitoring ec2 instances' status.
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by daemonk 11y ago
I guess there is value in the experience of purchasing a book as much as the book's content.
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by daemonk 11y ago
It looks like he has various sensors for gathering driving data. But how do you really know when you have gathered enough dimensions of data in this situation? How do you train for edge cases? I imagine there will still have to be some hard
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by daemonk 11y ago
Wow this is a great video. I was able to implement a simple python version of the BWT as described in the video here: https://gist.github.com/damiankao/908fe1fd6562dc75f40c
281.
by daemonk 11y ago
Decompressing takes 4 times as long? I wonder if that is slow enough to create a bottleneck in processing. Not everyone uses compression for purely archival purposes. In the genomics field, most sequencing data are gzipped to save disk spac
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by daemonk 11y ago
The interesting insight from the article is that python might be a good language for learning algorithms. The fast development time allows you to write a complete program (albeit clunky) without the pre-optimizing you might be tempted to do
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by daemonk 11y ago
Is there an absolute definition to these categories though? I would be pissed off too if I was a Muslim and the radical minority hijacked the terminology and twisted it into something different. But to the radical Muslims, their definition
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by daemonk 11y ago
They actually did something kinda clever. They were able to calculate/model a false positive rate by looking at the homozygosity of the X chromosome. Since the samples were male, there should only be one X, so any heterozygosity would
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by daemonk 11y ago
Our immune system is also full of somatic hyper-mutated cells.
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by daemonk 11y ago
I wonder if this is related to neurite repulsion. In Drosophla, the DSCAM gene is alternatively spliced to provide an "expression signature" for each neuron so they can maintain neurite repulsion. Maybe these point mutations are p
287.
by daemonk 11y ago
I am almost 2 years into my postdoc now. It's easy to be caught up in the academic bubble. Especially as a grad student. Things that are insignificant seems like a life or death decision for you. I know it is very difficult to get away
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by daemonk 11y ago
I am sure his reason for leaving academia is multi-faceted. His publication record (it's not that bad really. Maybe not competitive enough for a tenure track position) and his other scientific communication activities probably are part
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by daemonk 11y ago
There is an ideology that silicon valley stands for and the reality of the place. Those two things may be very disparate concepts, but that's another debate. I think the popular use of the silicon valley comparison is an attempt to get
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by daemonk 11y ago
"However, if you’re site doesn’t have those needs, CHOOSE SOMETHING ELSE!" "your" not "you're". Sorry I had to do it.
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by daemonk 11y ago
There are good reasons to not think trans-generational epigenetic inheritance is possible though. The main piece of evidence is the reprogramming that occurs after fertilization where epigenetic marks are erased and re-established. You coul
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by daemonk 11y ago
I don't know the research on this area very well. Does that mean for some people, their bodies will actually store the intake as fat preferentially even if the intake is a minimal amount for survival? You can actually die by eating a m
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by daemonk 11y ago
I don't quite understand how just using circ. RNAs would give you better predictions. From the little I've read about circ. RNAs is that they can form from normal RNAs and possibly function in sequestering regulatory small-RNAs. S
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by daemonk 11y ago
Bioinformatician here. When you say RNA testing, you mean RNA-seq? Gene expression? If yes, then do you think blood samples will be enough to make these type of diagnosis? I imagine you will probably need tissue specific samples to make som
295.
by daemonk 11y ago
Couldn't you use one of the available short read aligners (BWA,bowtie...) available for this also? Most of the aligners use some kind of FM-index for indexing the genome.
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by daemonk 11y ago
PAM sites? I am working on a genome assembly now and I want to try to identify potential g-rna sites around genic regions. This post is pretty helpful.
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by daemonk 11y ago
What is considered an "error"? Are there further classifications for different types of errors? Maybe there are certain error types that contribute more to allow you to detect bad servers? Seems like just having a single dimension
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by daemonk 11y ago
Sure. DNA/histone modifications do seem to serve some kind of purpose in gene regulation. I just find it a bit amusing that people seem to hold these two specific mechanisms in such high esteem to invent a whole new term, "epigene
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by daemonk 11y ago
The "genetics" in epigenetics can actually be quite controversial. That is, how epigenetic markers are inherited isn't very clear. In terms of DNA methylation, there are methylation maintenance enzymes. But for histone modifi
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by daemonk 11y ago
Great game. I like the incremental discoveries of new obstacles (linked blocks for example) or new mechanisms (lateral movement to combine numbers).

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