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This looks awesome! Just watched the brief intro video, how do you guys calculate the off-target effects?
by dluan 11y ago
This looks awesome! Just watched the brief intro video, how do you guys calculate the off-target effects?
- vineetg 11y ago(Author of the blog post here) We use the scoring function published by Hsu et al[1], which most scientists seem to be using. This function takes into account both the number of mismatches and where they occur in the guide. There's a more readable version here: http://crispr.mit.edu/about http://crispr.mit.edu/about . [1] http://www.nature.com/nbt/journal/v31/n9/full/nbt.2647.html http://www.nature.com/nbt/journal/v31/n9/full/nbt.2647.html
- danieltillett 11y agoInteresting that indels are not considered. We found that with RNA/DNA hybridisation that the probes with indels bind with high efficiency [1]. 1. http://www.ncbi.nlm.nih.gov/pubmed/20649647 http://www.ncbi.nlm.nih.gov/pubmed/20649647