3 ms·
Editing the Software of Life, for Fame and Fortune
- noname123 11y agoHi, I'm coming across CRISPR articles on a regular basis and was curious if any professional molecular biologist wet-lab peeps can give any pointers for DIY hobbyists if it's possible to do any DIY CRISPR experiments at any of the DIYBio hackerspaces around. So I believe the CRISPR reagents/plasmids can ordered here: https://www.addgene.org/CRISPR/ https://www.addgene.org/CRISPR/ A very rough guide for designing the experiment is shown here: https://www.addgene.org/crispr/planning-your-experiment/ https://www.addgene.org/crispr/planning-your-experiment/ I'm curious if it's possible for a layman to design a simple experiment say to 1) introduce the photoflourescent gene to bacteria via DNA recombinant techniques and 2) then maybe knock out that gene later via CRISPR by inserting some arbitrary nucleotides into the coding sequence of the photoflourescent gene, with visual confirmation; 3) and then re-enable photoflourescence later by deleting those arbitrary base-pairs with visual confirmation. Would love to learn more and do some hands-on stuff since I'm hearing so much about CRISPR everyday!