3 ms·
Right, thanks for clarifying that bit. An individual run still has a much higher error rate, but the ability to do exponentially more runs results in a higher a
by jballanc 11y ago
Right, thanks for clarifying that bit. An individual run still has a much higher error rate, but the ability to do exponentially more runs results in a higher accuracy per "experiment". This is not that dissimilar from the direction that other technologies have taken when facing physical measurement limits (cryo-electron tomography comes to mind, for example).
- new299 11y agoYes, though there's the added complication of time to answer however. You can turn a Sanger run around relatively quickly (I'd guess hours). High throughput runs sequence many millions of fragments of DNA in parallel very slowly. The highest throughput runs take several days to complete. This means that it's there are still (rapidly diminishing) scenarios where Sanger sequencing makes sense. Because you don't need the throughput, and you want an answer quickly.