2 ms·
About 6 Gigabases per hour, somewhere in that ballpark. Probably something like the equivalent of the human genome project (which took >10 years and 3 billion U
by new299 11y ago
About 6 Gigabases per hour, somewhere in that ballpark. Probably something like the equivalent of the human genome project (which took >10 years and 3 billion USD in the 90s) every few days.
Error rates are in the region of 1 to 0.1% generally.
I've never really worked directly with capillary data but understand it's error rate in early bases is limited by the amplification step. Which would be ~0.0001% (1 in 10000).
That said there might be a coarse filter (based on signal intensity etc) that would identify a subpopulation of high-throughput reads which have a <0.1% error rate. But I don't believe I've seen that reported. I'd be interested in hearing from anyone who has thoughts on that though.
- i000 11y agoThe absolute error-rate is not really a problem if the errors are random and you have the budget to sequence enough independent fragments. Sequencing becomes tricky (both Sanger and next-gen) because of non-random recurrent artifacts associated with the sequencing method or molecular manipulations done to the DNA or RNA.