4 ms·
You may only be able to sequence 30% of a single cell's exome (not even its entire genome), so you will not have that level of certainty.
by dbcooper 11y ago
You may only be able to sequence 30% of a single cell's exome (not even its entire genome), so you will not have that level of certainty.
- gwern 11y agoSo grow the embryo further and sequence more of it, or draw upon the prior information about off-target rates to make the best use of 30% coverage and pick ones with inferred rates which are acceptably low. Or use even more CRISPR to get rid of existing mutations to maintain total mutation load at normal rates... Many options. (Really, all these comments sound like people trying to find an excuse for why it doesn't work, rather than consider how it could work.)