As I posted earlier: > there did not seem to be much/any effort to design their plasmid to minimise these off target mutations. It's important to note that th

As I posted earlier: > there did not seem to be much/any effort to design their plasmid to minimise these off target mutations. It's important to note that the off-target actions are a known problem, and that there are methods to minimise them. Much effort is needed to study and optimise this, of course!