Autoclaving components of the medium separately has been done since the 1970s. Here's a paper published by my dad in 1975 in the Proceedings of the National Aca

Autoclaving components of the medium separately has been done since the 1970s. Here's a paper published by my dad in 1975 in the Proceedings of the National Academy of Sciences. Look under Materials and Methods, Media. It's mentioned like it's no big deal. They had tried a number of combinations, this is what worked best for them. http://authors.library.caltech.edu/11146/1/SUTpnas75.pdf http://authors.library.caltech.edu/11146/1/SUTpnas75.pdf EDIT: My dad has Parkinsons now. It's great to see him light up and talk about his work.