But before that, does having a PCI-E tunnel mean that you have to turn off the USB protocol? Or can you use both at the same time? How many pins are necessary t

But before that, does having a PCI-E tunnel mean that you have to turn off the USB protocol? Or can you use both at the same time? How many pins are necessary to transmit a basic (no power, just data) PCI-E 1x? And 4x, which would be the minimum viable for gaming? I'm interested to know more about this PCI-E tunnel thing.