5 ms·

Non-invasive circulating cancer cell (CTC) screening from blood samples seems to have the most promise. (disclaimer: I run a project in this area)

Bikanta 12y ago
that is actually one area of applications we are exploring! I'd love to have a chat with you about it.
- dbcooper 12y ago
  Sounds great! I will have to try to contact you/Bikanta when I return from vacation.
ihodes 12y ago
Which project? CTC screening is fascinating. Could you please point me to some good introductory papers/reading?
- dbcooper 12y ago
  I am on vacation right now, without my literature archive in arms reach, but from recent publications you may be interested in the whole-exome single cell sequencing work from Lohr et al. http://www.nature.com/nbt/journal/v32/n5/abs/nbt.2892.html http://www.nature.com/nbt/journal/v32/n5/abs/nbt.2892.html There is also an overview editorial in that issue of Nature Biotechnology. http://www.nature.com/nbt/journal/v32/n5/full/nbt.2897.html http://www.nature.com/nbt/journal/v32/n5/full/nbt.2897.html I remember seeing reviews in Lab Chip and Nat Rev Clinical Oncology recently. http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/C3LC50644D#!divAbstract http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/C3LC50... http://www.nature.com/nrclinonc/journal/v11/n3/full/nrclinonc.2013.253.html http://www.nature.com/nrclinonc/journal/v11/n3/full/nrclinon... The introduction sections of those papers should give you plenty of references to read. It's a good idea to look into the devices/methods used to enrich CTCs from blood prior to screening; Toner's devices based (in part) on EPCAM expression, and the CellScreen device (size/morphology), in particular.
  - ihodes 12y ago
    Thanks!
rgejman 12y ago
Agreed, for now. Caveats TBD. In particular issues of access (e.g. astrocytomas, non-metastatic bladder) and identity (what does it mean to be a CTC? A known driver mutation? We know that some tissues are littered with such mutations...)
akehrer 12y ago
You should get in touch with the folks at Denovo Sciences. They're working on a platform for CTC capture and identification. http://denovosciences.com/ http://denovosciences.com/

Bikanta 12y ago

that is actually one area of applications we are exploring! I'd love to have a chat with you about it.

dbcooper 12y ago
Sounds great! I will have to try to contact you/Bikanta when I return from vacation.

ihodes 12y ago

Which project? CTC screening is fascinating. Could you please point me to some good introductory papers/reading?

dbcooper 12y ago
I am on vacation right now, without my literature archive in arms reach, but from recent publications you may be interested in the whole-exome single cell sequencing work from Lohr et al. http://www.nature.com/nbt/journal/v32/n5/abs/nbt.2892.html http://www.nature.com/nbt/journal/v32/n5/abs/nbt.2892.html There is also an overview editorial in that issue of Nature Biotechnology. http://www.nature.com/nbt/journal/v32/n5/full/nbt.2897.html http://www.nature.com/nbt/journal/v32/n5/full/nbt.2897.html I remember seeing reviews in Lab Chip and Nat Rev Clinical Oncology recently. http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/C3LC50644D#!divAbstract http://pubs.rsc.org/en/Content/ArticleLanding/2014/LC/C3LC50... http://www.nature.com/nrclinonc/journal/v11/n3/full/nrclinonc.2013.253.html http://www.nature.com/nrclinonc/journal/v11/n3/full/nrclinon... The introduction sections of those papers should give you plenty of references to read. It's a good idea to look into the devices/methods used to enrich CTCs from blood prior to screening; Toner's devices based (in part) on EPCAM expression, and the CellScreen device (size/morphology), in particular.
- ihodes 12y ago
  Thanks!

rgejman 12y ago

Agreed, for now. Caveats TBD. In particular issues of access (e.g. astrocytomas, non-metastatic bladder) and identity (what does it mean to be a CTC? A known driver mutation? We know that some tissues are littered with such mutations...)

akehrer 12y ago

You should get in touch with the folks at Denovo Sciences. They're working on a platform for CTC capture and identification. http://denovosciences.com/ http://denovosciences.com/