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They do disappear off of your feed in which case you'll never see them again. There is no concept of "unread" tweets, like there is with RSS.

• nnkh 13y ago
  You can scroll down. The latter is true.
  • Touche 13y ago
    > You can scroll down. Not if you check it once a day (or even once an hour depending on how many things you follow), it's gone by then.