4 ms·

Indeed I think we are in agreement. Definitely in the need for enough cs to understand your own limitations. That's actually quite a bit of cs, but then again

Indeed I think we are in agreement. Definitely in the need for enough cs to understand your own limitations. That's actually quite a bit of cs, but then again obtaining a Ph.D. can take a while, so I'm sure a little more time for extra study can be found :) And taking a look at your work... Are you really trying to create a high throughput electron microscopy workflow? Whoa! If you make electron microscopy even close to high throughput that would be awesome! But how much of the brain structure are you able to preserve during sample prep? For that matter, how quickly does brain structure degrade after death? Minutes, hours, days, weeks, months? Are you imaging the sections in a light microscope first? Correlating the light and electron results of the same slice in software? Freezing the sample in liquid nitrogen so as to freeze it faster then ice crystal can form? Man science is fun, too bad it's not a good way to make a living.

davi 17y ago
"Are you really trying to create a high throughput electron microscopy workflow?" Yes. We've increased acquisition rates by a factor of ~15-20x over what is available using commercially available TEM systems. We now have tens of terabytes of image data in the can, and when I'm not reading HN (argh!) I'm working on collating & analyzing these data. Light level microscopy preceded embedding of the material for EM, the anatomy is correlated between the two modalities, and the sample preparation method (this is of mouse brain) is by perfusion with an aldehyde mixture, so there is essentially no deterioration of the ultrastructure. So, I wonder what you do -- no home page in your profile -- but I do remember what it's like to make a living. I like biology better. :)
- biohacker42 17y ago
  20x? That's awesome! I used to work in a bioinformatics startup - tons of fun with cutting edge science. But the startup tanked, and I moved on to a high paying corporate software engineering job. But I hope to be back in bioinformatics before long.
  - davi 17y ago
    Thanks. :) It's been a long road, hope to get a paper out in the next ~6 months or so. Shoot me an email (address on my home page) if you ever want to be in touch outside of HN.

davi 17y ago

"Are you really trying to create a high throughput electron microscopy workflow?" Yes. We've increased acquisition rates by a factor of ~15-20x over what is available using commercially available TEM systems. We now have tens of terabytes of image data in the can, and when I'm not reading HN (argh!) I'm working on collating & analyzing these data. Light level microscopy preceded embedding of the material for EM, the anatomy is correlated between the two modalities, and the sample preparation method (this is of mouse brain) is by perfusion with an aldehyde mixture, so there is essentially no deterioration of the ultrastructure. So, I wonder what you do -- no home page in your profile -- but I do remember what it's like to make a living. I like biology better. :)

biohacker42 17y ago
20x? That's awesome! I used to work in a bioinformatics startup - tons of fun with cutting edge science. But the startup tanked, and I moved on to a high paying corporate software engineering job. But I hope to be back in bioinformatics before long.
- davi 17y ago
  Thanks. :) It's been a long road, hope to get a paper out in the next ~6 months or so. Shoot me an email (address on my home page) if you ever want to be in touch outside of HN.