4 ms·
The role of the TLR-4 and TLR-9 agonists; Are they essentially acting as adjuvants for your vaccine when delivered in conjunction with the peptide on the surfac
by 1457389 13y ago
The role of the TLR-4 and TLR-9 agonists; Are they essentially acting as adjuvants for your vaccine when delivered in conjunction with the peptide on the surface of the same microsphere?
Also, did you consider using nanospheres? Why PLGA?
EDIT: This is the bit I want some clarification on, from your white paper:
>These microspheres make the very small targets look large and threatening to the immune system, provoking an immune response after a single dose
EDIT2: Got my answer in the white paper, yes they are adjuvants. Still would like to know why PLGA and what the mechanism of how they appear more "threatening" is.
- reidrubsamen 13y agoThanks for the question! PLGA is the one of the most commonly implanted biodegradable materials in the world - primarily because it is used is used in dissolving suture (check out this link to very popular PLGA based suture material). Aston S, Rees T. Vicryl Sutures. Aesthetic Plastic Surgery 1977;1:5. The "threatening point" is just a way of describing what TLR-4 (MPLA) and TLR-9 (CpG) do. In particular, MPLA "looks like" bacterial cell wall and CpG "looks like" bacterial DNA to the immune system. Steinhagen F. TLR-based immune adjuvants. Vaccine, 2011: 12. We did not consider using nano spheres because we wanted to get as much payload as possible into the antigen presenting cells without requiring multiple phagocytosis events per cell.
- 1457389 13y agoGot it, thanks and all the best! I for one will be watching closely so keep the updates coming.