3 ms·

Based on the sequencing read archives for that publication[1], it looks like they used Helicos[2]. PacBio[3] is another form of it. [1] http://www.ncbi.nlm.nih

Based on the sequencing read archives for that publication[1], it looks like they used Helicos[2]. PacBio[3] is another form of it. [1] http://www.ncbi.nlm.nih.gov/sra?LinkName=pubmed_sra&from_uid=23803765 http://www.ncbi.nlm.nih.gov/sra?LinkName=pubmed_sra&from_uid... [2] http://en.wikipedia.org/wiki/Helicos_single_molecule_fluorescent_sequencing http://en.wikipedia.org/wiki/Helicos_single_molecule_fluores... [3] http://en.wikipedia.org/wiki/Single_molecule_real_time_sequencing http://en.wikipedia.org/wiki/Single_molecule_real_time_seque...

new299 13y ago
The Helicos stuff would have to be pretty old, they've been effectively out of business for some time (having filed for bankruptcy in 2012). The paper suggests they mostly used Illumina, with one guy using Helicos: "J.S. did ancient and modern DNA extractions and constructed Illumina DNA libraries for shotgun sequencing"..."X.W. did Illumina libraries on donkey extracts"..."A.S.-O. performed Illumina sequencing for the Middle Pleistocene".."Ji.M. and X.W. performed Illumina sequencing for the Middle Pleistocene and the donkey genomes at BGI".."J.F.T. headed true Single DNA Molecule Sequencing of the Middle Pleistocene genome" It's worth noting that while Illumina sequencing isn't true single molecule, you are getting a read signal from a single fragment of DNA.