Your answer is correct, in order to get any pictures, you typically have to shine light through a sample (unless it is fluorescent, then you can bounce light of

Your answer is correct, in order to get any pictures, you typically have to shine light through a sample (unless it is fluorescent, then you can bounce light off of it). Also, in order to stain the sample, it needs to be thin(in this case 20um).Also, for the high mag stuff, you cut the section even thinner (ultra thin sectioning, which is the machine behind the guy under the "path to uploads?" heading). To do this, you need to already work with a thin section. Overall, sectioning brains is a super long, kinda boring process;) Hence why I've watched (listened) to a LOT of netflix. If you guys are interested in seeing open source brain images, check out http://www.brain-map.org http://www.brain-map.org, it's a great resource working on something similar.