Depending on what exactly you want, you may be able to use a cell free translation system rather than transfecting a bacterial host (or yeast, or whatevever els

Depending on what exactly you want, you may be able to use a cell free translation system rather than transfecting a bacterial host (or yeast, or whatevever else). Short peptides can also be produced by solid-phase synthesis: there are automated machines (peptide synthesisers) that produce a peptide with any arbitrary sequence from the constituent amino acids.