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An extra difficulty with genome assembly is that DNA often has lots and lots of repeated junk sequences that can confuse the algorithms. I don't work with bioin
by ufo 14y ago
An extra difficulty with genome assembly is that DNA often has lots and lots of repeated junk sequences that can confuse the algorithms. I don't work with bioinformatics to know how they usually get around this though.
- njbooher 14y agoRepeats aren't necessarily junk (e.g. TAL Effectors http://en.wikipedia.org/wiki/TAL_effector#DNA_recognition http://en.wikipedia.org/wiki/TAL_effector#DNA_recognition). Resolving them requires long reads. PacBio is currently of interest as an alternative to Sanger sequencing for this, although the error rate of PacBio reads is a bit of an issue.
- apathy 14y agopacbio is dead, they just don't know it yet. BGI (or somebody, doesn't matter, BGI is just the obvious candidate) would need to buy 50 SMART sequencers a year just for PacBio to stay in business. That seems unlikely given the lower cost and complexity of Illumina and Life sequencers