4 ms·
> I would be more worried if the blotted area was different (the dark blob) Or if more than one blob is present (i.e. blobs at different molecular weights) for
by flobosg 4mo ago
> I would be more worried if the blotted area was different (the dark blob)
Or if more than one blob is present (i.e. blobs at different molecular weights) for a supposedly selective and specific antibody that should show exactly one blob on the blot.
> Now, if while preparing the images they needed to do some editorial choices
Editorial choices on raw scientific data are a big no-no.
- raverbashing 4mo ago> Editorial choices on raw scientific data are a big no-no. I don't think you can find a picture in an article that hasn't been photoshopped in one way or another (which is mostly ok as long as it is not misleading) Edit: TF's reply is interesting https://www.thermofisher.com/es/es/home/life-science/antibodies/invitrogen-antibody-validation/image-transparency-frequently-asked-questions.html https://www.thermofisher.com/es/es/home/life-science/antibod... Basically they say they are reviewing the images
- flobosg 4mo agoUsually, journals require raw, unmodified data to be deposited as supplementary information.
- raverbashing 4mo agoYes, as supplementary information (Also journals are usually more rigorous than marketing material)
- flobosg 4mo ago> Yes, as supplementary information Still part of the article.