3 ms·
It amplifies sequences that contain the two primer sequences on each end of the target. So if you had synthesized sequence XYZ with some mistakes like YZX, then
by biophysboy 8mo ago
It amplifies sequences that contain the two primer sequences on each end of the target. So if you had synthesized sequence XYZ with some mistakes like YZX, then you could target X and Z and purify.
You're correct that PCR has a limited max length, but it is longer and cheaper than vanilla DNA synthesis.
- bookofjoe 8mo agoKary B. Mullis Nobel Prize lecture Nobel Lecture, December 8, 1993 The Polymerase Chain Reaction https://www.nobelprize.org/prizes/chemistry/1993/mullis/lecture/ https://www.nobelprize.org/prizes/chemistry/1993/mullis/lect...