4 ms·
Curious why you do CRISPR when HR works great in yeast. If you are having trouble transforming, try spheroplasty.
by dnautics 10mo ago
Curious why you do CRISPR when HR works great in yeast.
If you are having trouble transforming, try spheroplasty.
- koeng 9mo agoAlready answered there: I’m using bakers yeast, not lab yeast (store-bought S cerevisiae). It’s not haploid, often it’s tetraploid. HR doesn’t guarantee homozygous transformation. Same answer for electroporation vs spheroplasty. I’ve found with wild yeasts or less tamed yeasts (pichia), sometimes just nuking the damn thing with kV will just work, whereas those chemical methods can be way more finicky. Time is money