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It's a bad idea for a bunch of reasons but you could always do it yourself: https://nanoporetech.com/products/sequence/minion https://nanoporetech.com/products/
by a_tartaruga 1y ago
It's a bad idea for a bunch of reasons but you could always do it yourself: https://nanoporetech.com/products/sequence/minion https://nanoporetech.com/products/sequence/minion
- leoh 1y agoWhy is a bad idea for a bunch of reasons?
- a_tartaruga 1y agoIt is an expensive research project. Actually doing this starting from no experience or tools you're probably looking at 6 months - 1 year and 10k. A little support from trained biologists would help a lot though. Using DIY tools and not being a trained biostatistician or whoever usually looks at these things you are very likely to face errors you don't know how to account for. I would guess the odds are high you'll encounter scary false positives for example. It sounds really fun though, something I've always wanted to do with more time.
- greazy 1y agoSuggesting ONT is like suggesting getting a cpu without mentioning everything else that's required. Even then, you'll need expertise in analysing the output reads. And EVEN then it's difficult to accurately assess variants.