3 ms·
I'm sorry, can you clarify what that means? You wrote each piece of data 100 times? what does the 1000 refer to?
by ipince 14y ago
I'm sorry, can you clarify what that means? You wrote each piece of data 100 times? what does the 1000 refer to?
- skosuri 14y agowe printed the dna microarray using agilent's ink jet process (agilent is a spin off of hp; imagine an inkjet with actg instead of cmyk). each spot on the array has many hundreds of thousands of molecules. after we cleave the dna off the array, we take a portion of that, amplify and the sequence it. the portion we took off we estimate ~100 molecules for every oligo we made (55,000). we the sequence to get >55 million reads (so 1000x coverage); but these are just averages, and the distribution varies. you can check out the supplement of the paper if you are interested.
- somesaba 14y agoI think it refers to 1000x coverage on read which is then averaged