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To sequence we used about ~100x synthetic coverage on average and ~1000x sequencing coverage; so that's a whole lot of coverage. even then we did have 10 bit e
by skosuri 14y ago
To sequence we used about ~100x synthetic coverage on average and ~1000x sequencing coverage; so that's a whole lot of coverage. even then we did have 10 bit errors, but all the data blocks were recovered
- ipince 14y agoI'm sorry, can you clarify what that means? You wrote each piece of data 100 times? what does the 1000 refer to?
- skosuri 14y agowe printed the dna microarray using agilent's ink jet process (agilent is a spin off of hp; imagine an inkjet with actg instead of cmyk). each spot on the array has many hundreds of thousands of molecules. after we cleave the dna off the array, we take a portion of that, amplify and the sequence it. the portion we took off we estimate ~100 molecules for every oligo we made (55,000). we the sequence to get >55 million reads (so 1000x coverage); but these are just averages, and the distribution varies. you can check out the supplement of the paper if you are interested.
- somesaba 14y agoI think it refers to 1000x coverage on read which is then averaged