5 ms·
To read the data out are they basically doing de novo assembly on the sequenced reads? How are they handling all of the errors in gene sequencing? How about a
by tocomment 14y ago
To read the data out are they basically doing de novo assembly on the sequenced reads? How are they handling all of the errors in gene sequencing? How about assembly errors? Long repeats?
- CreRecombinase 14y agoIf your strand length is less than or equal to the read length of your sequencer, and you have the address blocks at the start of every sequence, you don't really need to worry about assembly. Read depth and/or a checksum of some kind will take care of errors in sequencing, and with short strands or compression of some kind, long repeats aren't much of a problem either.
- skosuri 14y agowe don't ever assemble; we are reading 115bp (96bp data, 15bp address), and pair reads using seqprep to reduce errors; a few other things are done, but the basics is that we just take all reads at a particular barcode and call consensus by majority vote. 10 bit errors in 5.27e6